Quest Diagnostic Patient Service Centers and Quest in-office phlebotomists do not collect respiratory specimens, including those from patients suspected to have . Maximum ten (10) per order. Item Description: Copan Universal Transport Media (UTM-RT) Contains one transport container and two (2) standard sized swabs. Miscellaneous Body Fluids. During the 2020 SARS-Cov-2 pandemic, there has been an acute shortage of viral transport medium. Specimen Collection Transport media for viruses are especially important as they are more labile than other infectious agents. Transport the specimen to the Microbiology . Phosphate buffered saline could carry COVID-19 testing samples safely. Universal Transport Medium for Viruses, Chlamydia, Mycoplasma and Ureaplasma. Cold samples will not flow In conjunction with the analytical LoD data, these results suggest that the novel HDPCR method is equivalent to traditional RT-PCR for detection of SARS-CoV-2. Eur J Microbiol Immunol 2013 - Frickmann H. et al. The cookie is used to store information of how visitors use a website and helps in creating an analytics report of how the wbsite is doing. NASAL ASPIRATE or WASH: Originally, specimen collection for SARS-CoV-2 testing required a specialized Nasopharyngeal (NP) swab and Viral Transport Media (VTM). Twelve different UTM/VTM liquid samples were added at different dilutions to the extraction buffer, and 2 of 12 generated false-positive results. The general rule is that all body fluids should be collected in sterile containers, without transport media. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. UTM™ ADVANTAGE 8.13 Withdraw 100µL of medium for QC sample. BD™Universal Viral Transport System Adding More Flexibility for Viral Testing Flexible, Compatible and Uniquely Designed! Submit the swab in Viral Transport Medium or Universal Transport Medium. Place swabs in viral transport medium (33595, 33625). organisms that can be present with devices containing transport media. UTM ® is a FDA cleared collection and transport system suitable for collection, transport, preservation, and long-term freeze storage of clinical specimens containing viruses for viral molecular diagnostic testing, including COVID-19, chlamydia, mycoplasma or ureaplasma organisms. Acceptable for Group A Strep Culture. While we understand the significant nationwide supply . Remel M6™ transport medium was compared to commercial and standard transport media routinely used for the transport and maintenance of viral, Chlamydia, Ureaplasma, and Mycoplasma agents. Study design Collect bronchoalveolar lavage and specimens from normally sterile sites in a sterile, leak-proof container (37777). Good for 24-48 hrs. Store specimen at room temperature . However, VTM Kit maintains the originality of virus samples to the greatest extent, and is a cultured virus transport medium. Safe viral transport medium ready for COVID-19 samples. 1 nasopharyngeal (NP) swab collected in a Viral-Chlamydia-Mycoplasma transport media (VCM) or Universal Transport Media (UTM) tube. Performance Characteristics of Quest V-C-M Medium are validated with BD™ Universal Viral Transport Swabs only. Options for swabs and media are below. Inactivating Viral Transport Medium with Universal Compatibility. Viral Transport Medium, 100/pk. Collection Instructions. Tissue Specimens. The VTM that was urgently required needed to support viral replication, as well as other routine diagnostic approaches. A: No, the policies outlined in the COVID-19 Transport Media Policy only apply to certain transport media devices that can be used to transport certain clinical specimens that are tested by. The assay has a full-process negative control, positive control and internal control. Viral Transport Medium. The two major types of growth media are those used for cell culture, which use specific . Bring to the boil to dissolve the agar completely. 2. Supply order number: 93307 Double Polyester Dry Swab Use for Group A Strep Rapid Antigen Screen and Group A Strep Direct DNA Probe. NEST VTM Viral Transport Medium Disposable Samplers are used for collection, storage, and transportation of human nasopharyngeal virus samples | COVID-19 Testing Supplies - VTM Transport Tube for Nasal Swab . ||Fluid: Transfer 3 mL specimen to a sterile container. Materials Provided Available Products Materials Required but not Provided 2. Update: The US Food and Drug Administration has expanded its guidance to include additional viral transport media and swab materials that can be used for COVID-19 testing. The sample-containing medium should be transported at 4 °C to achieve optimum results concerning the preservation of rickettsial viability. Viral/Molecular Infectious Disease PCR Testing Collect specimens for PCR testing early in illness when viral shedding is maximal. Screw down the caps firmly on the completely filled bottles. Viral transport media: M4, VCM and UTM or equivalent. Do NOT add the flocked swab to the transport media. Intended use(s): Copan Universal Transport Medium (UTM-RT) System is intended for the collection and transport of clinical specimens containing viruses, chlamydiae, mycoplasma or ureaplasma from. reduce level of testing for non-COVID related . As the Coronavirus Disease (COVID-19) response continues, many laboratories are reporting difficulty obtaining viral transport media (VTM). Bronchial lavage/wash, tracheal aspirate, sputum: Submit in a sterile leak-proof container. transport media & swabs for test code 7305 draft viral / universal transport media & eswabs multiple specimens should be submitted on individual requisitions to ensure testing of each; submit separate sample for other tests such as respiratory pathogens. swab in Universal Transport Medium (UTM-RT) and transport at room temperature for up to 24 hours after collection or refrigerate. This preparation follows the standard CDC-recommended formulation and contains 2% FBS,100µg /mL Gentamicin, and 0.5 µg /mL Amphotericin in a Hanks Balanced salt solution base. Remel M6™ transport medium was compared to commercial and standard transport media routinely used for the transport and maintenance of viral, Chlamydia, Ureaplasma, and Mycoplasma agents. Clinical studies have shown CultureSwab EZ swabs provide excellent recovery of clinically relevant aerobic bacteria up to 48 hours after collection.5 . COPAN UTM®-RT transport medium for viruses, chlamydia, mycoplasma, and ureaplasma provides good results regarding storage and transport of rickettsiae. _gid: 0: 1 day: This cookie is installed by Google Analytics. We describe the preparation and validation testing of VTM for rapidly expanding diagnostic testing, where the capacity of the VTM to preserve viral . [Specifications] 20 servings / box. Refer to the appropriate Aptima assay package insert for proper specimen storage conditions. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. The non-inactivated VTM Kit is to retain the protein shell of the virus and the DNA or RNA of the virus nucleic acid, so as to ensure the integrity of the nucleic acid of the virus sample during the inspection process. Order now. Specimen must not be contaminated with 3C047N. 2% FBS Minimum Volume. If the medium exhibits a distinct blue color throughout, discard the tube.5 2. The full name of UTM is universal transport media, which is literally translated as universal transport media. Viral Transport Media (VTM) Definition A viral transport media (VTM) is a nutrient substance used to carry and maintain the viability of specimens (viruses) to a laboratory for the identification and further processing of the sample. Large pieces may be transported in dry sterile container, as long as the specimen will be moist when it arrives in the laboratory. In fact, this novel FDA cleared and CE IVD marked infectious disease sample collection, storage and transport system was specifically developed to overcome the short comings of standard viral transport media (VTM) and universal transport media (UTM). COPAN Diagnostics, Inc. Of the seven media, Richards viral transport, SPG, Virocult, and HH medium, followed closely by tryptose phosphate broth, best preserved HSV infectivity at both 4 degrees C and 22 degrees C. Analysis of decay rates of RSV revealed comparatively rapid decay in SPG and Virocult. Unacceptable media (including but not limited to): Media with guanidine-containing materials, 'molecular media' that indicates inactivation of pathogens and preservation of RNA/DNA, charcoal media, Amies gel swabs, Meridian Viral Transport Media, Beaver Nano-technologies cell-free DNA tube, PrimeStore MTM, CY-F003-40 viral transport media, reduce level of testing for non-COVID related . These do not inactivate microbes and can also inhibit molecular testing. Refer to . Nasopharyngeal swabs, Oropharyngeal swabs (e.g. Sterilize by autoclaving at 121°C for 15 minutes. Table 1: Manufacturers of FDA-Approved Swabs for Coronavirus Testing. Acceptable: Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal . Insert swab into container with Universal Transport Medium. ETM (transport media) Clean screw-capped container for unpreserved specimens. Write A Review. section below. Refer to the applicable assay package insert for appropriate Specimen Lysis Tubes and specimen types available for use with each assay. but at the same time it retains the viral nucleic acid, which can be used for molecular diagnosis, sequencing and Nucleic acid amplification testing. The sudden arrival of the COVID-19 pandemic placed significant stresses on supply chains including viral transport medium (VTM). Do not dilute in universal transport media (UTM). Copan Universal Transport Medium (UTM-RT) System is intended for the collection and transport of clinical specimens containing viruses, chlamydiae, mycoplasma or ureaplasma from the collection site to the testing laboratory. below: VIRAL TRANSPORT MEDIUM . Many different products have been used to meet the demands of large-scale diagnostic and surveillance testing. The results were equivalent or superior to the media in the comparison. 8.14 Label the bottle, see . Small pieces of tissue should be placed in viral transport media or sterile PBS. Respiratory Viruses Rapid Culture (2001504) offers faster turnaround time. UTM-RT (Universal Transport Media - Room Temperature) (Copan Diagnostics Inc.) • Collection and Transport system for viruses, chlamydiae, mycoplasma and ureaplasma at RT • Provided as stand alone medium tubes and Nylon flocked plastic shaft pernasal swabs in peel pouches or as collection kits with medium tube combined with Nylon Universal Transport medium is a collection and transport system suitable for collection, transport, maintenance and long-term freeze storage of clinical specimens , including chlamydia, mycoplasma or ureaplasma organisms. The company makes products for diagnostics and specimen collection, controlled environments, environmental sampling . R64BX. That could fix a testing bottleneck due to viral transport medium shortages. Transport medium comparisons. It maintains organism viability for 48 hours at room or refrigerated temperature. Only one swab is required, however, if two swabs are used to sample the same source, the specimen is acceptable. Preferred Collection Media & Media Storage Storage after Collection Comments STOOL CULTURES Not performed on formed stool. UTM-RT can be . Universal Transport Medium. To test SARS-CoV-2 viral yield from different transport mediums over time, SARS-CoV-2 was serially diluted into plain DMEM for concentrations of 5.5 × 10 3 and 5.5 × 10 1. Request a Quote. For collection, transport, maintenance and long term freeze storage of Virus, Chlamydia, Mycoplasma and Ureaplasma SAFE, SHATTERPROOF, STAND UP tubes with distinctive internal conical shape enabling centrifugation of samples. Intended for the collection, transport and preservation of clinical specimens for viral molecular diagnostic testing. Virology » Lab Diagnosis of Viral Disease Viral Transport Media (VTM) are suitable for collection, transport, maintenance and long-term freeze storage of clinical specimens containing viruses, chlamydia, mycoplasma or ureaplasma organisms. multi-microbe and fecal transport products provide the confidence you need in specimen collection and transport. Remove swab and insert the swab into an acceptable viral transport medium . News provided by. This cookies is installed by Google Universal Analytics to throttle the request rate to limit the colllection of data on high traffic sites. PrimeStore® MTM is a molecular transport media that gives the user a "snapshot" in time for that sample by preserving and stabilizing DNA and RNA. Additionally, UTM-RT can be used for transport of swab specimens submitted for detection of bacterial (except B pertussis) or viral DNA by NAA (eg, PCR). As a trusted supplier of a broad range of high quality media and diagnostic products used in clinical, industrial, research, and academic laboratories, and backed by a team of service experts, we are dedicated to microbiology. COPAN announces a kit that pairs 3mL of UTM® universal viral transport medium and two FLOQSwabs™: one minitip and one regular flocked swab. The percentage of recovery for each agent when stored at 2-8°C was as follows: Preparation of Amies Transport Medium. [Product Use] Collection, storage and transportation of human nasopharyngeal virus samples. Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further . The percentage of recovery for each agent when stored at 2-8°C was as follows: The . The solution contains inorganic salts, amino acids, and protein-stable ingredients. 2. . Specimen Lysis Tubes and Specimen Transport Medium (STM) are intended to be used for processing specimens for use with Hologic® assays. Maintain at 4° C before and during transport to the laboratory. PrimeStore MTM (Molecular Transport Medium) was designed and optimized for molecular testing allowing pathogenic samples to be collected, transported, and processed safely and efficiently. Medium includes protein for stabilization, antibiotics to minimize bacterial and fungal contamination, and a buffer to maintain a neutral pH. Suspend 20g in 1 litre of distilled water. It provides a viral transport medium and a transport for the aforemontioned organisms in one system. We . To mitigate the supply issues, recommendations are two-fold: 1.Reduce demand for swab usage by: using a single swab for sampling throat then nose. throat swabs), sputum, saliva, and more can be stored in the reagent, then directly processed using extraction kits and workflows. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact . Break end of swab so top of vial can be screwed on securely. Preparation of Viral Transport Medium Page 4 of 8 SOP# DSR-052-05 . Detects common respiratory viruses as well as HSV and VZV. † Room temperature and refrigerated viability in ONE formulation † Compatible transport medium for the BD Directigen™EZ viral tests † Designed with safety and convenience to meet your viral testing needs The cobas® SARS-CoV-2 Test is a single-well dual target assay, which includes both specific detection of SARS-CoV-2 and pan-Sarbecovirus detection for the Sarbecovirus subgenus family that includes SARS-CoV-2. The transport media is a solution that keeps the specimen stable until it can be tested by the laboratory. 8.12 Cap the bottle securely and mix thoroughly by inverting the bottle. Preserve your options Multiple organisms in one medium To test SARS-CoV-2 viral yield from different transport mediums over time, SARS-CoV-2 was serially diluted into plain DMEM for concentrations of 5.5 × 10 3 and 5.5 × 10 1. . place each specimen in an individual transport bag and DO NOT refrigerate. place each specimen in an individual transport bag and The success of the diagnosis of SARS-CoV-2 (2019-nCoV) during the COVID-19 outbreak depends largely on the quality of the specimen and the conditions under which the specimen is transported and stored before being processed in the laboratory. The laboratory method performed depends on the type of media that the swab is placed in, e.g. Sterility Check and QC: Sterility Check A: Bulk Product . Sputum - Have the patient rinse the mouth with water and then expectorate deep cough directly into a sterile, leak-proof, screw-cap sputum collection cup or a sterile dry container. Respiratory viruses: Nasopharyngeal flocked swabs in universal transport medium are the preferred specimen type for respiratory virus testing. Differences between throat and NP swabs: Throat swabs usually have a straight shaft of uniform thickness with a thicker head than an NP swab. Specimen Transport and Storage. Amies transport medium is a widely used and effective semisolid medium for the transportation of swab specimens to the microbiology laboratory. 1 swab. Catalog no. Order now. Amies is a modification of Stuart's medium in which glycerophosphate is replaced by an inorganic . Subsequently, 500 μL of each virus dilution was then placed into separate sterile reagent reservoir trays (Corning Incorporated, 4870). Stuart Transport Medium will undergo a slight degree of oxidation indicated by a blue color at the upper periphery of the medium. Clear semi-solid/solid media is gel and will be rejected. Rating (required) 1 Star: 2 Stars: 3 . BBL™ Port-A-Cul™ Transport Systems BD™ Universal Viral Transport System . Molecular methods may offer improved sensitivity. Two kit sizes accommodating either 192 or 480 . COVID-19 specimen collection guidelines Preferred COVID-19 specimen transport media and swabs for Quest test code 39433 Listed in Quest Test Directory and Quanum™ eLabs/Lab Services Manager Reduce Preparation Steps. Swabs and transport media should only be used for testing conducted at DFS PHL. Universal Transport Medium (UTM) | Quidel Universal Transport Medium (UTM) By using UTM to transport your specimen, you maintain the capability to reflex negative samples to the diagnostic technology of your choice. The objective of this study was to determine the stability of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) RNA in specime … Processing: BAL or BW Specimen MUST be received in Microbiology laboratory within 2 hours of receipt in the RRL. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss Physcomitrella patens. the swab in a tube that is commonly filled with liquid (media) which maintains the sample for transport to the lab. Parafilm or tape the connection and lid of the sterile trap before transport. Direct nasal, throat or nasopharyngeal swabs should be tested as soon as possible after collection. The global coronavirus (CoV) disease 2019 (COVID-19) pandemic has resulted in a worldwide shortage of viral transport media and raised questions about specimen stability. The objective of our study was to compare PrimeStore® MTM at room temperature to universal transport media (UTM) shipped on dry ice in the detection of respiratory viruses in rural Honduran children less than 5 years of age. If nasopharyngeal (NP) swabs are needed, they must be ordered separately. In response to this VTM shortage, CDC has posted a new standard operating procedure (SOP) for laboratories to create their own VTM in accordance with CDC's protocol. Appropriately label specimen and send to the laboratory. Sodium glycerophosphate is a buffer; however, some organisms metabolize glycerophosphate with resultant promotion of bacterial growth.5 The laboratory will lyse the cells to recover either intact virus or viral nucleic acid and identify the virus through various laboratory methods. Place the swab into a vial of universal transport media (UTM). Subsequently, 500 μL of each virus dilution was then placed into separate sterile reagent reservoir trays (Corning Incorporated, 4870). In the virus sampling tube, it refers to the virus transport media. Adenovirus was stable in all media and at both temperatures tested. Includes a universal transporting medium that is room temperature stable, which can sustain viability (and infectivity) of a plurality of organisms during transit to the testing laboratory. To mitigate the supply issues, recommendations are two-fold: 1.Reduce demand for swab usage by: using a single swab for sampling throat then nose. NEW YORK - The latest bottleneck to confront the US healthcare system in rolling out molecular diagnostic testing for COVID-19 has been a shortage of tools and equipment needed to collect and transport samples. Request a Quote. fda is issuing this guidance to help facilitate the availability of devices for use in transporting certain clinical specimens, including transport media that can be used to transport certain. Multitest swab specimens must be transported to the laboratory in the provided swab specimen transport medium and tube. Importantly, this encompassed 9 different transport media, suggesting no discernable impact of media type when using extracted nucleic acid template. Distribute into small, screwcap bottles, stirring meanwhile to keep the charcoal evenly suspended. UTM™: UNIVERSAL TRANSPORT MEDIUM OFTEN IMITATED, NEVER REPLICATED. transport media & swabs for test code 7305 draft viral / universal transport media & eswabs multiple specimens should be submitted on individual requisitions to ensure testing of each; submit separate sample for other tests such as respiratory pathogens. Important: Due to revised guidance on transport media and in accordance with CAP/CLIA guidance, only media listed as acceptable in this guide will be accepted across the entire Quest Diagnostics enterprise. Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. The impact of universal transport media (UTM) and viral transport media (VTM) liquid samples on the performance of the Healgen Scientific Rapid COVID-19 Antigen Test was investigated. [Description] The virus sampling kit is based on Hank's solution. Materials and methods 2.1. Placing swabs in a moist container or transport medium prevents drying and the death of bacteria. Viral Transport Medium. V-C-M Medium is intended for use as a collection and transport medium for viral, chlamydial, mycoplasmal and ureaplasmal agents only.